Journal of Research in Medical Sciences

ORIGINAL ARTICLE
Year
: 2018  |  Volume : 23  |  Issue : 1  |  Page : 59-

Association of a disintegrin and metalloproteinase with a thrombospondin type 1 motif member 13 polymorphisms with severity of coronary stenosis in type 2 diabetes mellitus


Supakanya Lasom1, Nantarat Komanasin2, Nongnuch Settasatian3, Chatri Settasatian4, Upa Kukongviriyapan5, Pongsak Intharapetch6 
1 Biomedical Science Program, Graduate School; Cardiovascular Research Group, Khon Kaen University, Khon Kaen, Thailand
2 Cardiovascular Research Group; Department of Clinical Microscopy, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen, Thailand
3 Cardiovascular Research Group; Department of Clinical Chemistry, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen, Thailand
4 Cardiovascular Research Group; Department of Pathology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand
5 Cardiovascular Research Group; Department of Physiology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand
6 Cardiovascular Research Group; Queen Sirikit Heart Center of the Northeast, Khon Kaen University, Khon Kaen, Thailand

Correspondence Address:
Dr. Nantarat Komanasin
Department of Clinical Microscopy, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen 40002
Thailand

Background: The imbalance of von Willebrand factor (vWF) and a disintegrin and metalloproteinase with a thrombospondin type 1 motif member 13 (ADAMTS13) has been associated with atherosclerosis progression. A high level of vWF which regulates thrombus formation is associated with diabetes mellitus (DM), and some ADAMTS13 and vWF polymorphisms have effects on their levels. Therefore, this study aimed to evaluate the associations of ADAMTS13 and vWF polymorphisms and their levels with DM and severity of coronary stenosis. Materials and Methods: Eighty-seven DM and 84 control individuals were recruited. vWF and ADAMTS13 activities as well as vWF antigen were measured by collagen-binding assay (CBA), residual-CBA, and in-house enzyme-linked immunosorbent assay, respectively. ADAMTS1 3 and vWF polymorphisms were determined by polymerase chain reaction-restriction fragment length polymorphism. Results: The E and G alleles and AA genotype of ADAMTS13 Q448E, rs2073932, and rs652600, respectively, were independently associated with DM (odds ratio [OR] [95% confidence interval (CI)] = 2.5 [1.1, 5.6], 2.3 [1.0, 5.2], and 4.7 [1.2, 18.6], respectively). Moreover, E allele and AA genotype of Q448E and rs652600 were also significantly associated with multi-vessel disease (OR [95% CI] = 2.2 [1.0, 4.8] and 3.2 [1.0, 10.0], respectively), while the E and G allele of Q448E and rs2073932 were associated with high Gensini score (OR [95% CI] = 2.3 [1.1, 4.9] and 2.3 [1.1, 5.1], respectively). Conclusion: Association of ADAMTS13 polymorphisms with DM, number of vessel stenosis, and Gensini score may indicate the possible contribution of ADAMTS13 polymorphisms to atherosclerosis progression and severity of coronary stenosis in DM.


How to cite this article:
Lasom S, Komanasin N, Settasatian N, Settasatian C, Kukongviriyapan U, Intharapetch P. Association of a disintegrin and metalloproteinase with a thrombospondin type 1 motif member 13 polymorphisms with severity of coronary stenosis in type 2 diabetes mellitus.J Res Med Sci 2018;23:59-59


How to cite this URL:
Lasom S, Komanasin N, Settasatian N, Settasatian C, Kukongviriyapan U, Intharapetch P. Association of a disintegrin and metalloproteinase with a thrombospondin type 1 motif member 13 polymorphisms with severity of coronary stenosis in type 2 diabetes mellitus. J Res Med Sci [serial online] 2018 [cited 2021 Jul 25 ];23:59-59
Available from: https://www.jmsjournal.net/article.asp?issn=1735-1995;year=2018;volume=23;issue=1;spage=59;epage=59;aulast=Lasom;type=0