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β-Eudesmol induces the expression of apoptosis pathway proteins in cholangiocarcinoma cell lines
Chisato Narahara1, Teerachat Saeheng1, Wanna Chaijaroenkul2, Shyam Prakash Dumre3, Kesara Na-Bangchang4, Juntra Karbwang5
1 Department of Clinical Product Development, Institute of Tropical Medicine, Nagasaki University, Nagasaki, Japan
2 Graduate Studies, Chulabhorn International College of Medicine, Thammasat University, Pathumthani, Thailand
3 Department of Immunogenetics, Institute of Tropical Medicine, Nagasaki University, Nagasaki, Japan
4 Graduate Studies; Center of Excellence in Pharmacology and Molecular Biology of Malaria and Cholangiocarcinoma, Chulabhorn International College of Medicine, Thammasat University, Pathumthani, Thailand
5 Department of Clinical Product Development, Institute of Tropical Medicine, Nagasaki University, Nagasaki, Japan; Center of Excellence in Pharmacology and Molecular Biology of Malaria and Cholangiocarcinoma, Chulabhorn International College of Medicine, Thammasat University, Pathumthani, Thailand
Correspondence Address:
Prof. Juntra Karbwang
Department of Clinical Product Development, Institute of Tropical Medicine, Nagasaki University, 1-12-4 Sakamoto, Nagasaki 852-8523
Prof. Kesara Na-Bangchang
Center of Excellence in Pharmacology and Molecular Biology of Malaria and Cholangiocarcinoma, Chulabhorn International College of Medicine, Thammasat University, Pathumthani; Rangsit Center, Klong Luang, Pathumthani 12121
Thailand
 Source of Support: None, Conflict of Interest: None  | Check |
DOI: 10.4103/jrms.JRMS_291_19
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Background: Cholangiocarcinoma (CCA) is a neglected disease prevalent in developing countries with high burden and mortality rate, and there is no effective treatment. We aimed to investigate β-eudesmol molecular target of action in human CCA cell lines using the selected key molecules of apoptotic pathways. Materials and Methods: Two CCA cell lines (HuH28 and HuCCT1) were assessed at different time points after β-eudesmol treatment for mRNA and protein expression profiles of caspase-3, -8, -9, p53, p21, Bcl-2, and Bax by real-time polymerase chain reaction and western blot, respectively. Results: β-eudesmol induced expressions of p21 and p53 in mRNA/protein level in HuH28 and HuCCT1 cells. These CCA cells also expressed caspase-3, -8, -9 and bax (mRNA and/or protein level) among others after β-eudesmol treatment indicating its role in both intrinsic and extrinsic caspase-dependent apoptotic pathways. Conclusion: The study demonstrated that β-eudesmol induced the expression of apoptosis pathway proteins, suggesting its potential role in promoting the caspase-dependent apoptotic pathway, and induction of the cell cycle arrest in CCA cell lines. β-eudesmol can be considered as a potential compound for further investigation as an anti-CCA agent.
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