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J Res Med Sci 2020,  25:40

Comparison of multiple blood pressure frequency methods with optimum blood pressure measurement among Iranian individuals

1 Heart Failure Research Center, Isfahan Cardiovascular Research Institute, Isfahan University of Medical Sciences, Isfahan, Iran
2 Nursing and Midwifery Care Research Center, School of Nursing and Midwifery, Isfahan University of Medical Sciences, Isfahan, Iran
3 Isfahan Endocrine and Metabolism Research Center, Isfahan University of Medical Sciences, Isfahan, Iran
4 Interventional Cardiology Research Center, Isfahan Cardiovascular Research Institute, Isfahan University of Medical Sciences, Isfahan, Iran

Date of Submission15-Apr-2019
Date of Decision17-Jun-2019
Date of Acceptance03-Feb-2020
Date of Web Publication13-Apr-2020

Correspondence Address:
Dr. Davood Shafie
Heart Failure Research Center, Isfahan Cardiovascular Research Institute, Isfahan University of Medical Sciences, Isfahan
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Source of Support: None, Conflict of Interest: None

DOI: 10.4103/jrms.JRMS_129_19

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Background: Proper blood pressure (BP) measurement frequency method is less evaluated to optimize hypertension (HTN) status among different nations due to cultural patience variations. The aim of this study was to compare the first (BP1), second (BP2), and the mean of first and second (BP1,2) and all the three (BP1,2,3) with the second and third BP measurements in Iranian individuals. Materials and Methods: This cross-sectional study was done on 2264 individuals aged 18 years or more living in Isfahan city, Iran. Their BPs were assessed from right arms by the standard tools and methods after 5 min of rest in a quiet room by a trained staff. The mean second and third readings were considered as reference, and the other aforementioned variables were compared with it. Results: The mean age of total population was 40.9 ± 16.1 years (males: 52%). Men had significant higher means of systolic BP (SBP) and diastolic BP (DBP) with any measurement frequency methods than women (P < 0.001 and P = 0.009). Considerable clinical significant (≥5 mmHg) ranges were mostly observed in BP1compared with BP2,3. SBP2and DBP2indices showed insignificant differences compared with reference mean. Moreover, abnormal BP levels (≥140/90 mmHg) were mostly observed in terms of BP1measurement with no remarkable variability in BP2reading in comparison to the reference. Conclusion: Our considerable data suggested that BP2could appropriately categorize BP status similar to BP2,3and it may be rational for physicians considering this mean and excluding the first BP measurement as a sole criterion for HTN assessment in Iranian adults. Multiple researches are necessary quantifying appropriate frequencies of BP reading.

Keywords: Average, blood pressure, frequency, hypertension, mean

How to cite this article:
Mohammadi-Dolatabadi A, Vakhshoori M, Eghbali-Babadi M, Heidarpour M, Shafie D, Garakyaraghi M, Khosravi A. Comparison of multiple blood pressure frequency methods with optimum blood pressure measurement among Iranian individuals. J Res Med Sci 2020;25:40

How to cite this URL:
Mohammadi-Dolatabadi A, Vakhshoori M, Eghbali-Babadi M, Heidarpour M, Shafie D, Garakyaraghi M, Khosravi A. Comparison of multiple blood pressure frequency methods with optimum blood pressure measurement among Iranian individuals. J Res Med Sci [serial online] 2020 [cited 2022 Jan 22];25:40. Available from: https://www.jmsjournal.net/text.asp?2020/25/1/40/282337

*Alireza Mohammadi-Dolatabadi and Mehrbod Vakhshoori two authors have contributed equally to this work

  Introduction Top

Among noncommunicable diseases, the most common leading cause of death in both developed and developing nations are cardiovascular diseases (CVDs). It had been reported that of 422.7 million cases of CVDs, 17.92 million deaths occurred worldwide due to these diseases from 1990 till 2015.[1] These kinds of diseases cause a great governmental economic burden. For example, the annual direct cost in China was US$ 26.1 billion.[2] Several modifiable and nonmodifiable risk factors have been announced to be effective in CVDs occurrence, including hypertension (HTN), diabetes mellitus (DM),[3] dyslipidemia, smoking, obesity, lack of exercise, age, male gender, and positive family history.[4],[5] Among them, the leading contributor is HTN and several studies proved this entity as playing a pivotal role in CVDs' prevalence and mortality.[6],[7] Its prevalence and burden were predicted to be 32.3% and US$ 73.4 billion, respectively.[4],[8] In Iran, HTN prevalence was ranged from 17.3% to 22% based on different study population.[9],[10] One important factor existing to categorize persons to hypertensive or normotensive groups is blood pressure (BP) measurement, in which multiple methods have been available these days including ambulatory, home, and office BP monitoring with their own advantages and disadvantages.[11],[12],[13] Either underestimation or overestimation in BP measurements could cause complications related to longstanding HTN or extra costs and undesired medication side effects, respectively.[3],[14] Furthermore, usual office BP measurements give a poor estimation of individuals' BP because they often measure just one or two times apart.[15] Several guidelines have been made to improve BP measurement accuracy and focus mostly on sitting position or usages of appropriate-sized cuff.[14] Although most of them announced neglecting the first measurement due to its higher level and average the next two ones, there is no general consensus is the frequency of BP measurements.[15],[16] To best of our knowledge, there are few studies evaluating the number of BP measurements in each single office visit. For instance, a cross-sectional study done by Oladipo and Adedokun revealed that an average of five readings was excessive as compared to fewer ones to measure accurate BP.[16]

With respect to these inconsistencies and considering the point that appropriate BP measurement frequency might have some association with cultural properties of nations in terms of individuals patience, in this article, we aimed to compare the first (BP1), second (BP2) and the mean of first and second (BP1,2) and all three (BP1, 2, 3) BP measurements with the average of second and third BP readings as a reference group to define the most proper BP measurement frequencies for accurate HTN diagnosis among Iranian individuals.

  Materials and Methods Top

Study participants

This cross-sectional study was performed on individuals living in the third biggest city in central part of Iran, named Isfahan. From August 2015 till March 2016, anyone aged at least 18 years residing in Isfahan city was eligible for the recruitment in our study. Multi-stage random cluster sampling method was used selecting individuals who met inclusion criteria. Detailed methodology of that study published elsewhere.[17] Chosen individuals were invited to the nearest health care clinic. Written consent form was signed by each participant initially, and while they were seated, demographic information was asked. After that, BP was assessed from each individual's both arms three times with 1-min interval in which right arm BPs were considered for further analysis. Any limitations for measuring BP including arm casting or presence of fistula or shunts in hands or irregular pulses in addition to a history of fasting or being on special diet for weight control at the time of measurement, presence of chronic kidney disease, cancer of any kinds, mental illness, Cushing syndrome, and pheochromocytoma or being pregnant for female participants declaring by each person excluded subjects from the project. Finally, after merging all information, data of 2264 individuals were available to perform analyses. This study was approved by the Ethic Committee, affiliated to Isfahan University of Medical Sciences (IR.MUI.REC.2016.3.790).

Assessment of variables

A validated research-developed questionnaire was utilized gathering information about demographic factors. Questions about age, gender (male/female), education years (0–5, 6–12, and more than 12 years), marital status (single, married, and separated/widow), economic status (poor, moderate, and good), occupation (employed, unemployed, homemaker, student, and retired), and presence of DM, hyperlipidemia (HLP), and current smoking status (yes/no) were asked, while the participants were seated simultaneously. Then, participants were asked to take a rest for 5 min in a quiet room. BP measuring was performed according to the World Health Organization standard criteria in a way that participants were seated on a backed chair without their legs being crossed. Their feet soles were on the floor concurrently with their hands and palms at the heart level and in upward position, respectively.[18],[19] The equipment used for assessing BP was digital arm blood pressure (Microlife, Widnau, Switzerland). With 1-min interval, BP was measured three times from their right arms and all numbers were recorded.[17] To minimize the effect of white-coat HTN, personnel measuring BP did not wear white coats and their clothes were like the general population. Average of second and third BP means were defined as reference group and other measurements were calculated and declared as follows: first reading (BP1), second reading (BP2), average first and second readings (BP1,2), and average first, second and third readings (BP1, 2, 3). Height of participants was measured in centimeters with nonelastic measuring tape locating on the wall. Subjects' weights were assessed in kilograms (Kg) using a digital scale (Soehnle, Nassau, Germany) while they were asked to wear light-weight clothing and took of their shoes. Body mass index was assessed by division of weight in kilograms over height in meter square (Kg/m2). To increase variability and reliability of data collection, all participants' data were assessed with the same tool and by a fully trained personnel familiar how to work with aforementioned measuring instruments just after receiving an official letter from Isfahan Cardiovascular Research Institute. Furthermore, reading digital numbers were preferred compared with traditional hearing method. Calibration of tools including arm blood pressure, nonelastic height measurement tape, and digital scale was done via comparing with mercury sphygmomanometer verifying several times on 1–3 individuals, metal tape, and daily 5 Kg weight for control, respectively.

Statistical analysis

Categorical and continuous variables were reported as frequency (percentage) and mean ± standard deviation, respectively. Besides comparison of categorical variables with Chi-square test, comparison of numerical ones was done with independent t-test and analysis of variance plus paired t-test, as appropriate. All analyses were done using Statistical Package for the Social Sciences (SPSS) version 19 (IBM Corp., Armonk, NY, USA) and P < 0.05 was considered statistically significant.

  Results Top

The mean age of dominant male (52%) and female participants in our study was 41.3 ± 16.5 and 40.6 ± 15.8 years, respectively. General characteristics of individuals are provided in [Table 1]. Women were more obese and had more abnormal lipid levels, less educated, and mostly worked as homemakers. In contrast, men mostly worked as employees and smoked more frequently. There was no significant relation in terms of economic status and the presence of DM across genders.
Table 1: Distribution of general characteristics of study participants (n=2264)

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[Table 2] provides information about different BP measurements and their relation with reference BP category. Our data suggested that both systolic and diastolic blood pressures (SBP and DBP) were significantly higher in BP1 group in comparison to other readings including BP2, BP1,2, BP2,3, and BP1, 2, 3(P < 0.001 and P = 0.009, respectively). Although both BP1 indices and SBP1,2 and SBP1, 2, 3 showed significant differences from reference BP category, SBP and DBP of second measurement as well as DBP of other readings were not remarkably different with BP2,3 indices.
Table 2: Range of multiple blood pressure readings among study population and their relations with mean second and third blood pressure measurement

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Data about distribution of differences between reference BP measurement (BP2,3) and other readings (BP1, BP2, BP1,2 and BP1, 2, 3) are illustrated in [Table 3], revealing that most variabilities in other measurements remained in nonclinical spectrum in both BP indices, and clinically significant (>5 mmHg) difference from the reference mean BP category was mostly observed with measurement considering just the first reading (BP1).
Table 3: Differences between mean second and third blood pressure readings and other measurements ( first time blood pressure reading, second time blood pressure reading, mean first and second blood pressure readings, and mean first, second, and third blood pressure readings)

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[Table 4] shows differential of all measurements from a constant BP of 140/90 mmHg as well as their comparison with our predefined reference category. Comparing to the first time BP measurement, considering either second time BP or other mean readings (BP1,2, BP2,3, and BP1, 2, 3) led to categorization of more than 85% and 92% of individuals to SBP <140 and DBP <90 mmHg, respectively. The dominant percentage of abnormal BP (≥140/90 mmHg) was belonged to BP1 group. Further analysis revealed that both BP indices owning to BP1 were found to be more abnormal in comparison to the reference range. However, both indices of BP2 and BP1, 2, 3 as well as DBP1,2 categorized BP status like the reference mean (P > 0.05).
Table 4: Distribution of measurements relative to constant blood pressure of 140/90 mmHg and their relations with mean second and third blood pressure readings

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  Discussion Top

The aim of this study was to compare the first, the second, and the mean of first and second and all three BP measurements with the mean of second and third BP to find the most accurate method which appropriately categorizes individuals based on BP status, according to reference range among Iranian adults. HTN has been demonstrated to be one of the leading causes of CVDs. Therefore, proper HTN diagnosis contributes to decrease expenses and complications due to chronic HTN states or medication side effects in individuals mistakenly classified as normotensives or hypertensive, respectively. Furthermore, this point should be considered that individual patience is a potential factor might be effective in the quality of diagnostic and therapeutic management. Our findings suggested that while most of the BP differences from BP2,3 had been remained in nonclinical ranges (0–5 mmHg), more variations of at least 5 mmHg were mostly observed with BP measurement method considering the first time which also showed significant mean differences from the reference group. BP2,3 classified more than 90% of SBP and DBP in normal ranges, and the only measurement which showed significantly higher percentages of abnormal indices in comparison to the desired mean was BP1. Therefore, it might be reasonable to exclude first time BP measurement as a criterion for the assessment of person's HTN status. Moreover, our remarkable outcomes suggested that sole measuring of second BP indices could appropriately categorize HTN status like the reference range which is most widely used nowadays, especially in terms of lack of patience or possible annoyment of patients due to excessive BP measurements. To best of our knowledge, this was the first study in the literature comparing BP measurement frequency methods in Middle-Eastern population. Regarding to our results, there are few studies quantifying the proper number of office BP readings.[16],[20] For instance, Oladipo and Adedokun performed a cross-sectional study on 397 individuals aged at least 18 years. They measured BP for six times with 1-min interval in a single visit. After completion of the procedure, the first measurement had been discarded while other readings were categorized as desired means and compared with each other. Their results revealed that the five reading averages were numerously high for routine BP control in outpatient clinics; there was no significant alteration between the aforementioned mean and any other average readings such as the second and third ones. Quite small sample size and exclusion of the first BP measurement must be taken into account to generalize their findings.[16] A total of 83 individuals were recruited for the study and their BPs were assessed by two methods including usual office BP measurement (3 measurements with 30 s interval plus exclusion of the first reading) and 30-min one (11 readings every 3 min without considering the first one) repeating again 2 weeks later. Although their findings showed a better categorization of BP status with increased frequencies of measurements in the latter method comparing to usual office measurement, small sample size plus possible individual annoying due to high measurement frequencies and probable challenge for executing in actual settings should be considered.[20] In contrast, a study done by Kronish et al. on 707 healthy adults, in which their BPs were measured six times by two methods including mercury sphygmomanometer for the first three readings with 1–2 min interval and oscillometric device for the next three ones with 2 min interval though three distinct clinical visits with 1-week interval, showed that exclusion of the first BP reading did not cause any significant alterations in the estimation of true BP in neither two models. The order of BP measurement methods and the possibility of human error in manual were some limitations of this study.[3] Large sample size and the kind of BP measurement tool minimizing human error for recording were some of our advantages. Furthermore, personnel dressing looking like general population decreased the likelihood of white-coat HTN. This study was not free from limitations. Our measurements were taken by a digital equipment; therefore, the generalization of the findings to other reading methods including utilization of mercury sphygmomanometer or oscillometric tools should be cautiously done. Moreover, the location of BP measurement might be considered another limitation of the research. Finally, implementation of the current research in one city in Iran should be considered in terms of outcomes extension to other nations.

  Conclusion Top

Our results revealed that second BP measurement could classify individuals' HTN status similar to the reference reading and it may be reasonable to announce physicians for consideration of this mean as individual's true BP status in outpatient clinical visits and exclude the first time measurement. Several comprehensive studies required quantifying the exact frequency of BP measurements.


This study has been funded by the Iranian National Science Foundation and registered under number 91004751. The National Network of Cardiovascular Research and the Isfahan University of Medical Sciences as well as Isfahan Cardiovascular Research Institute have funded the study too. This article has been extracted from PhD thesis, No. 394,790 approved at Isfahan University of Medical Sciences.

Financial support and sponsorship

This study has been funded by the Iranian National Science Foundation and registered under number 91004751. The National Network of Cardiovascular Research and the Isfahan University of Medical Sciences as well as Isfahan Cardiovascular Research Institute have funded the study too.

Conflicts of interest

There are no conflicts of interest.

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  [Table 1], [Table 2], [Table 3], [Table 4]


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