Journal of Research in Medical Sciences

ORIGINAL ARTICLE
Year
: 2019  |  Volume : 24  |  Issue : 1  |  Page : 26-

Inhibition of hepatocellular carcinoma cell proliferation, migration, and invasion by a disintegrin and metalloproteinase-17 inhibitor TNF484


Changhong Xia1, Dongsheng Zhang2, Yanmei Li2, Jie Chen2, Haibo Zhou2, Long Nie2, Yanyan Sun2, Siyan Guo2, Jianbiao Cao3, Fangzheng Zhou2, Junlai Li1 
1 Department of Ultrasound, Chinese People's Liberation Army General Hospital, Beijing, China
2 Department of Oncology, Suizhou Hospital, Hubei University of Medicine, Suizhou, China
3 Department of Liver Disease, Army General Hospital of PLA, Beijing, China

Correspondence Address:
Dr. Junlai Li
Department of Ultrasound, Chinese People's Liberation Army General Hospital, No. 28 Fuxing Road, Beijing 100853
China

Background: The aim of this study was to test the effect of TNF484 on cell proliferation, migration, and invasion of hepatocellular carcinoma (HCC) cells. Materials and Methods: Various doses (0, 1, 10, 50, and 100 nM) of TNF484 were applied to the HepG2 and Bel7402 cells, and cell proliferation was measured by using 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyl tetrazolium bromide assay after 72 h. Cell migration rate was measured using the xCELLigence system, and the cell invasion ability was examined by the three-dimensional spheroid BME cell invasion assay. The expression level of ADAM17 was also measured with RT-PCR. Results: With the treatment of TNF484, the cell proliferation of HepG2 and Bel7402 cells was inhibited in a dose-dependent manner. Moreover, under TNF484 treatment, the cell migration rate as well as cell invasion ability of the HepG2 and Bel7402 cells were suppressed. Conclusion: TNF484 could inhibit the cell proliferation, migration, and invasion of some HCC cell lines, making it a potential therapeutic option for liver cancer treatment.


How to cite this article:
Xia C, Zhang D, Li Y, Chen J, Zhou H, Nie L, Sun Y, Guo S, Cao J, Zhou F, Li J. Inhibition of hepatocellular carcinoma cell proliferation, migration, and invasion by a disintegrin and metalloproteinase-17 inhibitor TNF484.J Res Med Sci 2019;24:26-26


How to cite this URL:
Xia C, Zhang D, Li Y, Chen J, Zhou H, Nie L, Sun Y, Guo S, Cao J, Zhou F, Li J. Inhibition of hepatocellular carcinoma cell proliferation, migration, and invasion by a disintegrin and metalloproteinase-17 inhibitor TNF484. J Res Med Sci [serial online] 2019 [cited 2019 Apr 20 ];24:26-26
Available from: http://www.jmsjournal.net/article.asp?issn=1735-1995;year=2019;volume=24;issue=1;spage=26;epage=26;aulast=Xia;type=0