Seminal plasma and CD4+ T-cell cytokine profiles in the in vitro fertilization success
Zahra Kanannejad1, Bahia Namavar Jahromi2, Behrouz Gharesi-Fard3
1 Department of Immunology, Shiraz University of Medical Sciences, Shiraz, Iran
2 Department of Gynecology and Obstetrics; Infertility Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
3 Department of Immunology; Infertility Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
|Date of Submission||02-May-2019|
|Date of Decision||25-Aug-2019|
|Date of Acceptance||14-Dec-2019|
|Date of Web Publication||18-Mar-2020|
Prof. Behrouz Gharesi-Fard
Department of Immunology, Shiraz University of Medical Sciences, Shiraz, P.O.Box: 71345-1798
Source of Support: None, Conflict of Interest: None
Background: Abnormal female immune response is one of the potential causes of unexplained infertility (UI). Seminal plasma (SP) is an important regulator of female immune responses during pregnancy. This study investigated a SP effect on the expression of CD4+ T-cell-related cytokines in a group of UI woman candidates for in vitro fertilization (IVF) and healthy fertile women. Materials and Methods: This was a semi-experimental study that performed on 20 UI couples (ten unsuccessful and ten successful IVF outcomes) and 10 fertile couples as the healthy group. CD4+ T-cells were separated from peripheral blood mononuclear cells of women by magnetic-activated cell sorting technique and incubated with (stimulated condition) or without (unstimulated condition) SP of their husbands. After incubation, real-time polymerase chain reaction method was used to investigate interleukin (IL)-23, IL-17, IL-4, IL-10, transforming growth factor (TGF)-β, and interferon (IFN)-γ gene expression. Mann–Whitney U-test, Kruskal–Wallis test, and Wilcoxon signed-rank test were used for statistical analysis. Results: Baseline TCD4+ mRNA levels of IL-23 (P = 0.03) and TGF-β (P = 0.01) were different between healthy and infertile groups. However, IL-17, IL-4, IFN-γ, and IL-10 were expressed similarly regardless of fertility status. Comparing mRNA expression before and after SP exposure, our results have shown that relative expression of IL-23 significantly increased in successful (P = 0.04) and unsuccessful IVF groups (P = 0.01), whereas IL-10 expression increased only in the IVF failure group (P = 0.01). Conclusion: SP can make a positive effect on IVF outcome through alteration in CD4+ T-cell-related cytokines expression, especially IL-10 and IL-23.
Keywords: CD4-positive T-lymphocytes, fertilization in vitro, infertility, real-time polymerase chain reaction, seminal plasma
|How to cite this article:|
Kanannejad Z, Jahromi BN, Gharesi-Fard B. Seminal plasma and CD4+ T-cell cytokine profiles in the in vitro fertilization success. J Res Med Sci 2020;25:26
|How to cite this URL:|
Kanannejad Z, Jahromi BN, Gharesi-Fard B. Seminal plasma and CD4+ T-cell cytokine profiles in the in vitro fertilization success. J Res Med Sci [serial online] 2020 [cited 2020 Jul 7];25:26. Available from: http://www.jmsjournal.net/text.asp?2020/25/1/26/280925
| Introduction|| |
Infertility is a failure of the reproductive system for achievement of a successful pregnancy after 1 year of regular unprotected intercourse. This condition has an effect on approximately 7%–15% of couples worldwide. The reasons for infertility remain unknown in 25% of infertile patients who belong to unexplained infertile group. The immune system has the most important effect on pregnancy outcome, and an immune imbalance was considered as the main reason for 50% of unexplained infertility (UI) cases. In vitro fertilization (IVF) is a potential treatment method for UI while the results of it are varied. Several studies have shown that poor response to ovarian stimulation and also some immunological factors are related to IVF failure.,
T-helper (Th) cells, which are one of the main immune cell subsets of the adaptive immune system, play an important role in pregnancy period. Four CD4+ T-cells subsets including Th1, Th2, Th17, and regulatory T-cells (Treg) are recognized as the main players of the immune system during pregnancy and also its health problems. In recent years, several studies have shown that immune hemostasis between Th cell subsets contributes to fertility and successful outcomes. Different Th cell subsets secrete distinct types of cytokines that act together as a regulatory pathway during successful pregnancy. Th1 cells secret some inflammatory cytokines including interferon (IFN)-γ and tumor necrosis factor (TNF)-α which play a significant role in inflammatory and cell-mediated immune responses. On the other hand, Th2 cells are included in anti-inflammatory and humoral immune responses by producing interleukin (IL)-4, IL-5, and IL-13. Evidence from studies on murine and human pregnancy indicates an association between successful pregnancy and Th2-type immune responses, while Th1 immune response is associated with pregnancy failure. Th17, a subset of CD4+ T-cells, is identified by expressing retinoic acid receptor-related orphan receptors (Rorc) and IL-17 production. Th17 cells are involved in many pregnancy disorders including preeclampsia and recurrent spontaneous abortion. Tregs, which originated from naive CD4+ T-cells under transforming growth factor (TGF)-β effect, are the main type of T-cells that are crucial for the maternal immune tolerance to the fetus. Treg disabilities are reported in UI, recurrent abortion, and also some pregnancy diseases such as preeclampsia and fetal growth restriction.
Successful implantation needs to some cellular and molecular changes in the uterine tissue. Some studies suggested that seminal plasma (SP) component such as TGF-β through providing the antigenic and environmental signals in uterine may have a critical role in successful implantation. In this line, some evidence showed that patients who received intravaginal SP treatment in the time of ovum pick-up or embryo transfer had higher implantation and clinical pregnancy rates compared to those who did not receive such treatment. SP components have an effect on the expression of a wide range of female immune factors such as some cytokines and chemokines that led to activation and regulation of the immune cells in the uterus., In a previous study, we have shown that SP might increase Th17 and Treg cell frequencies in infertile women with IVF failure and might also balance inflammatory to regulatory responses to finally tune-up the Th1/Th2/Th17/Treg balance and support the success of IVF. Furthermore, we have recently reported the SP effects on cytokine expression level which released by peripheral blood mononuclear cells (PBMCs). We showed that after SP exposure, the mRNA level of forkhead box protein 3 (Foxp3), Rorc, IL-23, IL-17, IL-6, TGF-β, and IL-35 increased in women with successful IVF outcome. In line with our previous studies, we aimed to elucidate the probable role of SP mediated by cytokine expression in success of fertilization following IVF treatment. To attain this goal, for the first time, the mRNA level of CD4+ T-cell-related cytokines was investigated before and after SP exposure in healthy and unexplained infertile women with successful and unsuccessful IVF outcomes.
| Materials and Methods|| |
This semi-experimental study was approved by the local Ethics Committee of Shiraz University of Medical Sciences (IR.SUMS.REC.1394.S632). All individuals signed informed consent. Individuals enrolled in the present study were selected from our subject's bank and were the same as our previous study.
Blood and semen samples were collected from 20 unexplained infertile couples who were candidates for IVF treatment as an infertile group and 10 fertile couples as a healthy group. Among infertile couples, 10 had successful IVF outcome and so were considered as a successful group and the remaining 10 had unsuccessful IVF outcome so were considered as an unsuccessful group. The infertile women who participated in this study had no intercourse 2 weeks before IVF treatment. No random allocation was performed in this study.
Moreover, all unexplained infertile couples were checked for history of previous positive pregnancy and immunological disorders at the time of sampling. Participants with such problems besides the men with male tract infections or varicocele were excluded from the study. The healthy fertile group was interviewed for the recorded history of fertility problems and selected if they had one previous termed pregnancy. Other excluding criteria were the same as the fertile group. Hormone level was measured for all women. Moreover, semen analysis test was performed for all male partners in the infertile and healthy groups.
Semen was obtained from all participating men. Fresh semen was liquefied for 30 min at 37°C and then centrifuged for 15 min at 3000 RPM (room temperature). The supernatant was collected and centrifuged again at 14,000 RPM for 30 min in 4°C to remove all remaining spermatozoa. Finally, SP was collected and stored at −70°C.
Peripheral blood mononuclear cell isolation
PBMCs were isolated from whole blood samples by a density gradient centrifugation method using Lymphoprep (Axis-Shield, Oslo, Norway) and then washed in RPMI 1640 media (Gibco by Thermo Fisher Scientific, New York, USA). Cells were counted and resuspended to cryovials containing freezing media and preserved in liquid nitrogen tank until the time of experiments.
Isolation and purification of CD4+ T-cells by magnetic-activated cell sorting
In our experiment, a negative selection strategy was used to separate CD4+ T-cells. The CD4+ T-cell isolation kit (Miltenyi Biotec, Bergisch Gladbach, Germany) with 95%–99% reproducibly was used for fast isolation of these cells from PBMCs. 8 × 106 PBMCs were resuspended in magnetic-activated cell sorting (MACS) buffer and CD4+ T-cell biotin-antibody cocktail (Miltenyi Biotec, Bergisch Gladbach, Germany). After incubation, the CD4+ T-cell microbead cocktail (Miltenyi Biotec, Bergisch Gladbach, Germany) was added to the mixture and incubated for 20 min in the refrigerator. Then, the cell suspension was applied onto the MS column (Miltenyi Biotec, Bergisch Gladbach, Germany) in order to achieve CD4+ T-cells. Approximately 47% of cells were positive for CD4 before MACS isolation while this percentage increased to 97% after MACS purification [Figure 1]. For each individual, the viability and the number of cells were up to 97% and 3 × 106, respectively. Subsequently, the cells were cultured in RPMI for SP stimulation.
|Figure 1: Purity of magnetic-activated cell sorting isolated T-cell. (a) CD3+CD4+ T-cell before and (b) after magnetic-activated cell sorting isolation|
Click here to view
A total of 1 × 106 female PBMCs/well were cultured without exposure to male SP (unstimulated condition or before intervention) and also co-cultured with SP (stimulated condition or after intervention) (0.3% V/V total concentration) in the 24-well plates in 1 ml RPMI 1640 media supplemented with 10% fetal bovine serum (Shell Max, China) and 1% penicillin-streptomycin (Shell Max, China) and incubated at 37°C in a humidified 5% CO2 incubator for 24 h. After culture, cells were collected and stored in −70°C until the time of experiments.
Real-time polymerase chain reaction
Cellular RNA was extracted from isolated CD4+ T-cells by RNA isolation kit (Pars tous, Mashhad, Iran). Then, the RNA was quantified by spectrophotometry (Eppendorf BioPhotometer D30 Hamburg, Germany). 150–200 ng of total RNA template was reversed transcript into cDNA by the ABI high-capacity cDNA reverse transcription kit (Thermo Fisher Scientific, Waltham, Massachusetts, USA) with a sensitivity of 0.02–0.2 μg, according to the manufacturer's protocol, and cDNA was stored at −70°C. SYBR Green Master Mix reagents (Applied Biosystems, Foster City, California, USA) was used for amplification of cDNAs by real-time polymerase chain reaction (PCR) technique using QuantStudio™ instrument (Applied Biosystems, Foster City, California, USA). Amplification program was same for all studied mRNAs: 95°C for 10 min (Hot start) and 45 cycles consisting of 95°C for 10 s, 60°C for 30 s, and 72°C for 30 s. The dissociation protocol was added after the final PCR cycles to assure specificity. The sequence of all corresponding primers was derived from related published studies., All used primers are summarized in [Table 1]. The ΔΔCt method, normalized target gene to RPL13a expression as a housekeeping gene, was applied to quantify the mRNA expression of IL-23, IL-17, IL-10, IL-4, IFN-γ, and TGF-β genes.
Statistical analysis was performed by SPSS software version 18 (SPSS Inc., Chicago, Illinois, USA). Mann–Whitney U-test and Kruskal–Wallis test were used for mean comparison between two or more than two groups, respectively. Wilcoxon signed-rank test was used for comparing two paired groups (stimulated and unstimulated conditions). P < 0.05 was considered as statistically significant.
| Results|| |
Unstimulated CD4+ cultures
Baseline CD4+ mRNA level (unstimulated condition) of IFN-γ, TGF-β, IL-4, IL-17, IL-23, IL-10, and RPL13a genes were assessed using SYBR Green real-time PCR method [Table 2]. The level of IL-23 and TGF-β was significantly different between the healthy fertile and infertile groups (P = 0.03, P= 0.01, respectively) [Table 2]. The expression of IL-23 (P = 0.008) decreased in successful IVF, whereas the expression of TGF-β (P = 0.01) decreased in women with unsuccessful IVF as compared with the healthy group [Table 2]. There are no significant differences in unstimulated levels of IFN-γ, IL-17, IL-10, and IL-4 among different infertile and healthy groups [Table 2].
|Table 2: Fold change in relative expression of cytokine in unstimulated CD4+ from infertility and fertile group|
Click here to view
The difference in the mRNA levels of CD4+ T-cell-related cytokines in seminal plasma stimulated and nonstimulated conditions
Mean fold change in cytokine expression after SP stimulation (stimulated condition) in comparison to unstimulated condition (calibrator) is presented in [Table 3]. There were no significant differences in the relative expression of cytokines in the healthy group after SP treatment. The relative expression of IL-23 significantly increased in the successful IVF group after SP stimulation (P = 0.04) while there were no significant differences regarding the expression of other cytokines in this group [Table 3]. The relative expression of IL-23 and IL-10 in the IVF failure group was significantly higher compared with an unstimulated condition (before treatment with SP) (P = 0.01 and 0.01, respectively).
|Table 3: Fold change in relative gene expression of cytokines in different studied groups before and after stimulation with seminal plasma|
Click here to view
| Discussion|| |
Previous studies showed that infertility might be associated with an inappropriate function of the female immune system. The adaptive immune response plays an important role in etiology of UI. In the first part of this study (baseline CD4+ mRNA level of cytokines in unstimulated condition), the results showed that IL-23 and TGF-β levels increased in the fertile group as compared to infertile ones. IL-23 induces the differentiation of naive CD4+ T-cells into Th17 cells producing IL-17A, IL-17F, IL-6, and TNF-α, which leads to pro-inflammatory processes and neutrophil-mobilization., In addition to inducing inflammatory responses, increasing local concentration of matrix metallopeptidase-9 and stimulating angiogenesis for implantation are induced by IL-23. In line with our results, recent evidence has shown that the proportion of Th17 cells and their related cytokines (IL-17 and IL-23) was higher in the first trimester than in the postnatal period. Furthermore, Th17 cells may be involved in the inflammatory process which is essential for successful implantation. However, some clinical studies showed that IL-23 had a destructive effect after implantation. A study by Darmochwal-Kolarz et al. showed that increased expression of IL-23 in the first trimester is associated with recurrent pregnancy losses. TGF-β has an important role in the proliferation and differentiation of trophoblast cells at the human feto-maternal interface. Evidence showed that TGF-β level, in trophoblast-stimulated PBMC cultures, was significantly lower in individuals who experienced failed pregnancy compared with normal pregnant women. Therefore, increased expression of IL-23 and TGF-β in the healthy group can be considered as positive factors in favor of a normal pregnancy.
In the second part of this study, we showed that SP stimulation can lead to increase in expression of IL-23 in the healthy and infertile groups, although these changes were not statistically significant in healthy couples. We previously showed that SP exposure might increase IL-23 level in PBMCs of infertile patients. Previous studies have shown that villous and extravillous trophoblast cells express IL-23 in early period of pregnancy, and this cytokine modulates the expression of other cytokines and also helps cell survival in endometrial cells. Therefore, increased expression of IL-23 by SP stimulation may be in favor of IVF outcome in the infertile patient.
Furthermore, our data indicated that SP stimulation could increase IL-10 expression in women with IVF failure. IL-10 is a pleiotropic anti-inflammatory cytokine with the ability to suppress the pro-inflammatory milieu. Increased expression of IL-10 during the first and second trimesters but not in the third trimester has been reported. Although IL-10 is not essential for the growth and development of the fetus, it has a main role in the inhibition of increased inflammatory responses. In line with our results, it is reported that SP fractions such as prostaglandin E2 (PGE2) and 19-hydroxy-PGE2 might stimulate IL-10 release from a monocytic cell line. Hence, increased IL-10 level after SP insertion might improve IVF outcome in women with unsuccessful IVF outcome. It is also reported that SP might induce a significant increase in intracellular expression of the immunosuppressive cytokine such as TGF-β in women with unsuccessful IVF outcome., In line with the mentioned studies, our data indicated that the level of TGF-β may increase after SP stimulation in women with unsuccessful IVF outcome, although not significant. The sample size can be considered as the major reason for this discrepancy and also the main limitation of the current study.
| Conclusion|| |
In summary, SP might have a positive effect on IVF outcome through changes in TCD4+-related cytokine levels, especially IL-10 and IL-23. However, we worked on a small population of infertile and healthy women, and a larger sample population should be provided for identifying certain effects of SP on IVF treatment outcome.
This study was extracted in part from the Ph.D. thesis written by Zahra Kanannejad and financially supported by Grant No. 94-7600 from Shiraz University of Medical Sciences.
Financial support and sponsorship
This study was supported by Shiraz University of Medical Research (Grant No: 94-7600).
Conflicts of interest
There are no conflicts of interest.
| References|| |
Hamada A, Esteves SC, Nizza M, Agarwal A. Unexplained male infertility: Diagnosis and management. Int Braz J Urol 2012;38:576-94.
Boes AS, De Neubourg D, Peeraer K, Tomassetti C, Meuleman C, D'Hooghe T. Unexplained infertility. Evid Based Obstet Gynecol 2019;9:145-53.
Ray A, Shah A, Gudi A, Homburg R. Unexplained infertility: An update and review of practice. Reprod Biomed Online 2012;24:591-602.
Pandian Z, Bhattacharya S. IVF for unexplained infertility. Hum Reprod Update 2013;19:431.
Adib Rad H, Basirat Z, Mostafazadeh A, Faramarzi M, Bijani A, Nouri HR, et al
. Evaluation of peripheral blood NK cell subsets and cytokines in unexplained recurrent miscarriage. J Chin Med Assoc 2018;81:1065-70.
Comins-Boo A, Garcia-Segovia A, Nunez P. Evidence-based update: Immunological evaluation of recurrent implantation failure. Reprod Immunol Open Acc 2016;1:1-8.
Saito S, Nakashima A, Shima T, Ito M. Th1/Th2/Th17 and regulatory T-cell paradigm in pregnancy. Am J Reprod Immunol 2010;63:601-10.
Piccinni MP, Lombardelli L, Logiodice F, Kullolli O, Romagnani S, Le Bouteiller P. T helper cell mediated-tolerance towards fetal allograft in successful pregnancy. Clin Mol Allergy 2015;13:9.
Osborne LM, Brar A, Klein SL. The role of Th17 cells in the pathophysiology of pregnancy and perinatal mood and anxiety disorders. Brain Behav Immun 2019;76:7-16.
Robertson SA, Care AS, Moldenhauer LM. Regulatory T cells in embryo implantation and the immune response to pregnancy. J Clin Invest 2018;128:4224-35.
Nikolaeva MA, Babayan AA, Stepanova EO, Smolnikova VY, Kalinina EA, Fernández N, et al
. The relationship of seminal transforming growth factor-β1 and interleukin-18 with reproductive success in women exposed to seminal plasma during IVF/ICSI treatment. J Reprod Immunol 2016;117:45-51.
Crawford G, Ray A, Gudi A, Shah A, Homburg R. The role of seminal plasma for improved outcomes during in vitro
fertilization treatment: Review of the literature and meta-analysis. Hum Reprod Update 2015;21:275-84.
Sharkey DJ, Tremellen KP, Jasper MJ, Gemzell-Danielsson K, Robertson SA. Seminal fluid induces leukocyte recruitment and cytokine and chemokine mRNA expression in the human cervix after coitus. J Immunol 2012;188:2445-54.
Guerin LR, Prins JR, Robertson SA. Regulatory T-cells and immune tolerance in pregnancy: A new target for infertility treatment? Hum Reprod Update 2009;15:517-35.
Kanannejad Z, Namavar Jahromi B, Gharesi-Fard B. T cell subsets profiling in unexplained infertile women with successful and unsuccessful in vitro
fertilization outcome: Focus on the effect of seminal plasma. Iran J Allergy Asthma Immunol 2019;18:163-72.
Azad M, Keshtgar S, Jahromi BN, Kanannejad Z, Gharesi-Fard B. T helper cell subsets and related cytokines in infertile women undergoing in vitro
fertilization before and after seminal plasma exposure. Clin Exp Reprod Med 2017;44:214-23.
Nogueira LG, Santos RH, Fiorelli AI, Mairena EC, Benvenuti LA, Bocchi EA, et al
. Myocardial gene expression of T-bet, GATA-3, Ror-γt, FoxP3, and hallmark cytokines in chronic Chagas disease cardiomyopathy: An essentially unopposed TH1-type response. Mediators Inflamm 2014;2014:914326.
Ledderose C, Heyn J, Limbeck E, Kreth S. Selection of reliable reference genes for quantitative real-time PCR in human T cells and neutrophils. BMC Res Notes 2011;4:427.
Cai JY, Li MJ. Interleukin 23 regulates the functions of human decidual immune cells during early pregnancy. Biochem Biophys Res Commun 2016;469:340-4.
Li J, Chen X, Liu Z, Yue Q, Liu H. Expression of Th17 cytokines in skin lesions of patients with psoriasis. J Huazhong Univ Sci Technolog Med Sci 2007;27:330-2.
Darmochwal-Kolarz D, Michalak M, Kolarz B, Przegalinska-Kalamucka M, Bojarska-Junak A, Sliwa D, et al
. The role of interleukin-17, interleukin-23, and transforming growth factor-beta in pregnancy complicated by placental insufficiency. Biomed Res Int 2017;2017:6904325.
Lissauer D, Goodyear O, Khanum R, Moss PA, Kilby MD. Profile of maternal CD4 T-cell effector function during normal pregnancy and in women with a history of recurrent miscarriage. Clin Sci (Lond) 2014;126:347-54.
Saito S, Nakashima A, Ito M, Shima T. Clinical implication of recent advances in our understanding of IL-17 and reproductive immunology. Expert Rev Clin Immunol 2011;7:649-57.
Ginsburg ES, Xiao L, Gargiulo AR, Kung FT, Politch JA, Schust DJ, et al
. T-helper 2 and 3 type immunity to trophoblast in successful in vitro
fertilization-embryo transfer. Fertil Steril 2005;83:1659-64.
Uz YH, Murk W, Yetkin CE, Kayisli UA, Arici A. Expression and role of interleukin-23 in human endometrium throughout the menstrual cycle and early pregnancy. J Reprod Immunol 2010;87:21-7.
Cheng SB, Sharma S. Interleukin-10: A pleiotropic regulator in pregnancy. Am J Reprod Immunol 2015;73:487-500.
Meuleman T, Snaterse G, van Beelen E, Anholts JD, Pilgram GS, van der Westerlaken LA, et al
. The immunomodulating effect of seminal plasma on T cells. J Reprod Immunol 2015;110:109-16.
Robertson SA, Guerin LR, Bromfield JJ, Branson KM, Ahlström AC, Care AS. Seminal fluid drives expansion of the CD4+CD25+T regulatory cell pool and induces tolerance to paternal alloantigens in mice. Biol Reprod 2009;80:1036-45.
Robertson SA, Prins JR, Sharkey DJ, Moldenhauer LM. Seminal fluid and the generation of regulatory T cells for embryo implantation. Am J Reprod Immunol 2013;69:315-30.
[Table 1], [Table 2], [Table 3]