Effects of nanozeolite/starch thermoplastic hydrogels on wound healing
Hossein Salehi1, Mohammad Mehrasa2, Bijan Nasri-Nasrabadi3, Mohsen Doostmohammadi3, Reihaneh Seyedebrahimi1, Navid Davari4, Mohammad Rafienia5, Mehdi E Hosseinabadi6, Maria Agheb7, Mansour Siavash8
1 Department of Anatomical Sciences, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
2 Department of Biotechnology, Faculty of Advanced Sciences and Technologies, University of Isfahan, Isfahan, Iran
3 Department of Chemical Engineering, Isfahan University of Technology, Isfahan, Iran
4 Department of Medical Sciences, School of Medicine, Shahrekord University of Medical Sciences, Shahrekord, Iran
5 Biosensor Research Center, Isfahan University of Medical Sciences, Isfahan, Iran
6 Department of Biomedical Engineering, Faculty of Engineering, University of Isfahan, Isfahan, Iran
7 Research Centre of Faculty of Advanced Technologies, Isfahan University of Medical Sciences, Isfahan, Iran
8 Isfahan Endocrine and Metabolism Research Center, Isfahan University of Medical Sciences, Isfahan, Iran
Isfahan Endocrine and Metabolism Research Center, Isfahan University of Medical Sciences, Isfahan
Source of Support: None, Conflict of Interest: None
Background: Wound healing is a complex biological process. Some injuries lead to chronic nonhealing ulcers, and healing process is a challenge to both the patient and the medical team. We still look forward an appropriate wound dressing.
Materials and Methods: In this study, starch-based nanocomposite hydrogel scaffolds reinforced by zeolite nanoparticles (nZ) were prepared for wound dressing. In addition, a herbal drug (chamomile extract) was added into the matrix to accelerate healing process. To estimate the cytocompatibility of hydrogel dressings, fibroblast mouse cells (L929) were cultured on scaffolds. Then, 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium-bromide assay test and interaction of cells and scaffolds were evaluated. For evaluating healing process, 48 male rats were randomly divided into four groups of four animals each (16 rats at each step). The ulcers of the first group were treated with the same size of pure hydrogels. The second group received a bandage with the same size of hydrogel/extract/4 wt% nZ (hydrogel NZE). The third group was treated with chamomile extract, and the fourth group was considered as control without taking any medicament. Finally, the dressings were applied on the chronic refractory ulcers of five patients.
Results: After successful surface morphology and cytocompatibility tests, the animal study was carried out. There was a significant difference between starch/extract/4 wt% nZ and other groups on wound size decrement after day 7 (P < 0.05). At the clinical pilot study step, the refractory ulcers of all five patients were healed without any hypersensitivity reaction.
Conclusion: Starch-based hydrogel/zeolite dressings may be safe and effective for chronic refractory ulcers.